Journal: STAR Protocols

Article Title: A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

doi: 10.1016/j.xpro.2020.100182

Figure Lengend Snippet:

Article Snippet: 48-pin spotter (frogger) , V&P Scientific Inc. , Cat. #: VP 407AH.

Techniques: Software, Spectrophotometry

Journal:

Article Title: Mutagenesis of SNM1 , Which Encodes a Protein Component of the Yeast RNase MRP, Reveals a Role for This Ribonucleoprotein Endoribonuclease in Plasmid Segregation

doi:

Figure Lengend Snippet: Growth phenotypes of yeast strains carrying a mutant snm1 gene. Two colonies from each mutant yeast strain were mixed with 100 μl of sterile double-distilled H2O in the first-row wells of a 96-well Falcon tissue culture plate; 10-fold serial dilutions of these cell mixtures were placed in the second-, third-, and fourth-row wells. A 48-pin Frogger was used to transfer diluted cell mixtures to SCD plates. The cells were grown for 2 days at 24, 30, and 37°C and for 4 days at 17°C. SNM1 alleles are listed in Table ​Table1.1. All SNM1 alleles are carried on LEU2 CEN vector pRS315 (51) in strain THR200. WT, wild type.

Article Snippet: A 48-pin Frogger (Dan-kar Corp.) was used to transfer diluted cell mixtures to SCD plates.

Techniques: Mutagenesis, Plasmid Preparation